CRV Lagoa, the largest insemination central of Latin America, provides breeders all over Brazil with sexed semen.
The most modern laboratory of bovine sexed semen in the country was inaugurated on September 23, 2005 in Sertãozinho (SP)headquarters. The unit received investments of R$ 6 million and uses the technology of Sexing Technologies (USA), one of the most respectful organizations of animal reproduction in the world, present in USA and Colombia. The minimum birth expectation of the chosen sex is 85%.
The sexing semen represents the most advanced stage in the evolution of animal reproduction, after the traditional techniques such as embryos transference, “in vitro” fertilization, sexing and bipartition of embryos.
The laboratory constructed at CRV Lagoa has the capacity for 12 machines. Production at present is of about 10 thousand doses per month, with the power to reach 30 thousand. All the crew that work at the lab was trained at Sexing Technologies in the United States, and capable of operating and maintaining the machines.
Before starting work, thousands of sexed semen doses of CRV Lagoa were tested with the utmost care in Brazilian cattle breeding farms with better results than those that had been guaranteed.
Frequently Asked Questions:
1 – What is sexed semen?
It is semen separated by sex in populations with chromosomes X (female) and Y (male).
2 – How is semen purified?
By means of a flow cytometer (MoFlo SX). Bovines have a difference of 3.8 % on average of the total DNA weight between chromosomes X and Y.
3 – Who developed this technique?
Dr Larry Johnson of the United States Department of Agriculture (USDA), in 1976.That department is the owner of the main patent of this technique, through the company XY Inc.
4 – How does flow citometry work?
A small laser beam lights the cells individually, in an extremely thin stream. Detectors analyze the scattered light emitted by the fluorochrome attached to the DNA cells. Cells are recognized and selected.
5 – How many doses per ejaculate can be produced?
4 to 10 doses per hour. That depends on many factors such as:
● The bull ( 80% to 90% bull semen is suitable for sorting);
● the ejaculate quality: this factor influences both the speed and purity process.
6 – What is the sexed semen purity for the chosen sex?
Generally the purity is of 92%; the minimum acceptable is 85%. However, it depends on the ejaculate quality.
7 – How many spermatozoa are there in one dose of sexed semen?
Researches at the XY Inc. and at Colorado University clearly demonstrate that there are no fertility differences between doses of 2 and 10 million spermatozoa.
CRV Lagoa commercializes doses of ¼ cc (thin straw) for:
● Artificial Insemination with 2 million spermatozoa;
● ET/IVF with 5 million spermatozoa.
8 – How effective in conception is sexed semen?
That always depends on the bull, but in tests conducted in the USA and in Brazil, on the average:
● AI: 15% percentage point lower than conventional semen (at the most);
● IVF: near conventional semen;
● ET: near conventional semen (batches with concentration of 5 million spermatozoa per straw).
9 – When was born the first heifer from frozen sexed semen?
10 – What are the guarantees and safety of sexed semen?
The sexing lab follows the same biosafety patterns as those already established for the conventional semen laboratory of CRV Lagoa. The guarantee is of a minimum of 85% for the chosen sex. Sexed semen must receive the same attention paid to conventional semen concerning storage, handling and application.
11 - What is sexed semen packaging like?
Sexed semen for use in IA is packaged in thin pink straws for female and blue for males. Semen used in ET/IVF is packaged in yellow (female).
12 – What are the advantages of using sexed semen on the herd?
● Freedom to choose the sex of the calf and define the course of your business;
● Increase number of females or males;
● More selection intensity, accelerating herd genetic improvement;
● Herd internal replacement with genetic quality and sanitary safety;
● Optimization of wanted genetic characteristics;
● Reduction of distocic deliveries, with the use of female sexed semen that are usually lighter at birth ( mainly in milking herds).